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Date: 12-2-2020
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Date: 15-2-2020
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Since Kc is a factor that is wholly dependent on a particular column and solvent flow rate, a quantitative measure of the affinity of a compound for a particular set of mobile and stationary phases that does not depend on the column geometry is useful. The retention factor, k, can be derived from Kc and is independent of the column size and the solvent flow rate.
The retention factor is calculated by multiplying the distribution constant by the volume of stationary phase in the column and dividing by the volume of mobile phase in the column.
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