Aneuploidy and Aneusomy

As introduced in Chapter 5, cytogenetic changes are hallmarks of cancer, whether sporadic or familial, particularly in later and more malignant or invasive stages of tumor development. Constitutional chromosomal abnormalities also predispose to cancer, as in Down syndrome (acute lymphoblastic leukemia and acute myeloid leukemia), Turner syndrome (germ cell tumors), and Klinefelter (germ cell tumors, breast cancer). Somatic cytogenetic alterations suggest that a critical element of cancer progression includes defects in genes involved in maintaining chromosome stability and integrity and ensuring accurate mitotic segregation.

Initially, most of the cytogenetic studies of tumor progression were carried out in leukemias because the tumor cells were amenable to being cultured and karyotyped by standard methods. For example, when CML, with the t(9;22) Philadelphia chromosome, evolves from the typically indolent chronic phase to a severe, life- threatening blast crisis, there may be several additional cytogenetic abnormalities, including numeric or structural changes, such as a second copy of the 9;22 translocation chromosome or an isochromosome 17q. In advanced stages of other forms of leukemia, other translocations are common. In contrast, a vast array of chromosomal abnormalities is seen in most solid tumors. Cytogenetic abnormalities found repeatedly in a specific type of cancer are likely to be driver events involved in the initiation or progression of the malignant neoplasm. A current focus of cancer research is to develop a comprehensive cytogenetic and genomic definition of these abnormalities, many of which result in enhanced proto- oncogene expression or the loss of TSG alleles. Medulloblastoma is a salient example of this comprehensive molecular and cytogenetic characterization for which four distinct types are described: SHH, Wnt, Group 3, and Group 4. Each has characteristic clinical features and distinct treatment- related outcomes. Genome sequencing is replacing cytogenetic analysis in many instances because it provides a level of sensitivity and precision well beyond detection of cytologically visible genome changes. Furthermore, RNA (cDNA) fusion detection utilizing NGS is also commonly deployed to detect somatic oncogenic fusions, and technologies now exist that enable detection of fusions without prior knowledge of the fusion partner or translocation breakpoints.

Gene Amplification

In addition to translocations and other rearrangements, another cytogenetic aberration seen in many cancers is gene amplification, a phenomenon in which many additional copies of a segment of the genome are present in the cell (see Fig. 1). Gene amplification is common in cancers, including neuroblastoma, squamous cell carcinoma of the head and neck, colorectal cancer, and malignant glioblastomas of the brain. Amplified segments of DNA are readily detected by comparative genome hybridization or genome sequencing and appear as two types of cytogenetic change in routine chromo some analysis: double minutes (very small accessory chromosomes) and homogeneously staining regions that do not band normally and contain multiple, amplified copies of a particular DNA segment. How and why double minutes and homogeneously staining regions develop are poorly understood, but amplified regions are known to include extra copies of proto- oncogenes such as the genes encoding Myc, Ras, and epithelial growth factor receptor, which stimulate cell growth, block apoptosis, or both. For example, amplification of the MYCN proto- oncogene encoding N- Myc is an important clinical indicator of prognosis in the child hood cancer neuroblastoma. MYCN is amplified more than 200- fold in 40% of advanced stages of neuro blastoma; despite aggressive treatment, only 30% of patients with advanced disease survive 3 years. In contrast, MYCN amplification is found in only 4% of early- stage neuroblastoma, and the 3- year survival is 90%. Amplification of genes encoding the targets of chemo therapeutic agents has also been implicated as a mechanism for the development of drug resistance in patients previously treated with chemotherapy.

Fig1. Different mutational mechanisms leading to proto- oncogene activation. These include a single point mutation leading to an amino acid change that alters protein function, mutations or translocations that increase expression of an oncogene; a chromosome translocation that produces a novel product with oncogenic properties; and gene amplification leading to excessive amounts of the gene product.

اخر الاخبار

اخبار العتبة العباسية المقدسة

العتبة العباسية المقدسة تنظم المحاضرة الثالثة من محفل نهج البلاغة في مقام الإمام المهدي (عجّل الله فرجه)

اللجان المشرفة على حفل التكليف الشرعي تطلع على استعدادات مجمع أبي الفضل العباس (عليه السلام)

باختبارات نظرية وعملية.. المجمَع العلمي يختتم دورة أحكام التلاوة في النجف الأشرف

شركة خير الجود: نشارك في مهرجان عين الحياة لدعم المواطن بمنتجات ذات جودة عالية

المزيد

الآخبار الصحية

الشاي والقهوة وتدهور الذاكرة.. دراسة تكشف نتائج جديدة

هل يساعد الزنك على تحسين النوم والطاقة؟

علامات يجب عدم تجاهلها بعد تناول مكملات "فيتامين د"

لتجنب ارتفاع سكر الدم.. ما أفضل وقت لتناول الحلوى؟

المزيد

الآخبار التكنلوجية

الإمارات ترسخ مكانتها كأحد أبرز قادة الذكاء الاصطناعي عالميا !

"أوبن إيه آي" تحذر المشرعين الأميركيين من تحايل "ديب سيك" ؟

تصاعد التوتر بين "أبل" وإدارة ترامب.. ما السبب؟

سلاح طبي في ساحات الحرب.. رذاذ يوقف النزيف خلال ثانية !

المزيد

مواضيع ذات صلة

Telomerase as an Oncogene

Activation of Oncogenes by Chromosome Translocation or Fusions

Imaging of the Pituitary: Adenomas

T pit Family of tumours

Pit- 1 Family of tumours

Pituitary Adenomas

Molecular Mechanisms of Carcinogenesis

Chronic Myeloid Leukemia, BCR-ABL Gene, and Disease Monitoring

Application of Liquid Biopsy to Non-small- Cell Lung Cancer Lung

Circulating Tumor DNA

Biomarkers in the Blood and in Tissues: A Complementary Approach

Biomarkers in Oncology: An Evolving Concept