Holliday Junctions Must Be Resolved

KEY CONCEPTS
- The bacterial Ruv complex acts on recombinant junctions.
- RuvA recognizes the structure of the junction.
- RuvB is a helicase that catalyzes branch migration.
- RuvC cleaves junctions to generate recombination intermediates.

- Resolution in eukaryotes is less well understood, but a number of meiotic and mitotic proteins are implicated.
One of the most critical steps in recombination is the resolution of the Holliday junction, which determines whether there is a reciprocal recombination or a reversal of the structure that leaves only a short stretch of hybrid DNA . Branch migration from the exchange site  determines the length of the region of hybrid DNA (with or without recombination).
The proteins involved in stabilizing and resolving Holliday junctions have been identified as the products of the ruv genes in E. coli. RuvA and RuvB increase the formation of heteroduplex structures. RuvA recognizes the structure of the Holliday junction. RuvA binds to all four strands of DNA at the crossover point and forms two tetramers that sandwich the DNA. RuvB is a hexameric helicase with an ATPase activity that provides the motor for branch migration. Hexameric rings of RuvB bind around each duplex of DNA upstream of the crossover point. A diagram of the complex is shown in FIGURE 1.

FIGURE 1. RuvAB is an asymmetric complex that promotes branch migration of a Holliday junction.
The RuvAB complex can cause the branch to migrate as fast as 10 to 20 bp per second. A similar activity is provided by another helicase, RecG. RuvAB displaces RecA from DNA during its action. The RuvAB and RecG activities both can act on Holliday junctions, but if both are mutant, E. coli is completely defective in recombination activity.
The third gene, ruvC, encodes an endonuclease that specifically recognizes Holliday junctions. It can cleave the junctions in vitro to resolve recombination intermediates. A common tetranucleotide sequence provides a hotspot for RuvC to resolve the Holliday junction. The tetranucleotide (ATTG) is asymmetric, and thus may direct resolution with regard to which pair of strands is nicked. This determines whether the outcome is patch recombinant formation (no overall recombination) or splice recombinant formation (recombination between flanking markers). Crystal structures of RuvC and other junction-resolving enzymes show that there is little structural similarity among the group, in spite of their common function.
We may now account for the stages of recombination in E. coli in terms of individual proteins. FIGURE 2. shows the events that are involved in using recombination to repair a gap in one duplex by retrieving material from the other duplex. The major caveat in applying these conclusions to recombination in eukaryotes is that bacterial recombination generally involves interaction between a fragment of DNA and a whole chromosome. It occurs as a repair reaction that is stimulated by damage to DNA, but this is not entirely equivalent to recombination between genomes at meiosis. Nonetheless, similar molecular activities are involved in manipulating DNA.

FIGURE 2. Bacterial enzymes can catalyze all stages of recombination in the repair pathway following the production of suitable substrate DNA molecules.
All of this suggests that recombination uses a “resolvasome” complex that includes enzymes catalyzing branch migration as well as junction-resolving activity. It is possible that mammalian cells contain a similar complex.
Although resolution in eukaryotic cells is less well understood, a number of proteins have been implicated in mitotic and meiotic resolution. S. cerevisiae strains that contain mus81 mutations are defective in recombination. Mus81 is a component of an endonuclease that resolves Holliday junctions into duplex structures. The resolvase is important both in meiosis and for restarting stalled replication forks . Other proteins known to be involved in the resolution process are described in the broader context of eukaryotic homologous recombination factors in the following section.

اخر الاخبار

اخبار العتبة العباسية المقدسة

قسم الإعلام يشارك في الاجتماع التحضيري لمناقشة الخطة الإعلامية لزيارة عاشوراء

بحضور متوليها الشرعي.. العتبة العباسية المقدسة تواصل إقامة المجالس الحسينية لإحياء شهر المحرم

قسم الشؤون الفكرية يطلق برنامجه التبليغي في أكثر من 20 دولة إفريقية

شعبتا الخطابة النسوية والتوجيه الديني تواصلان إقامة المجالس الحسينية في مرقد أبي الفضل العباس (عليه السلام)

المزيد

الآخبار الصحية

دراسة تكشف علاقة خطيرة بين عمر الأم وصحة مولودها

دراسة تحذر.. اضطراب نوم بسيط قد يسبب الموت المبكر ؟

الاستحمام بالماء الساخن.. راحة مؤقتة وأضرار دائمة للبشرة

ماذا يحدث للجسم عند تخطي وجبة الإفطار؟

المزيد

الآخبار التكنلوجية

كيف تساعد الصيانة الوقائية في الحفاظ على كفاءة التكييف في سيارتك ؟

في ظاهرة غريبة حيرت العلماء.. كوكبنا قد يسجل أقصر يوم في التاريخ هذا الصيف!

السد الصيني العملاق يعيق دوران الأرض ويغيّر شكلها ؟

السعودية تطلق 10 تجارب علمية إلى محطة الفضاء الدولية

المزيد

مواضيع ذات صلة

How AraC Protein Loops and Unloops

DNA Looping and Repression of araBAD

Binding Sites of the ara Regulatory Proteins

Endoplasmic Reticulum-Associated Degradation and the Unfolded Protein Response

Protein Folding and Modifications in the Endoplasmic Reticulum

Protein Modifications

Regulating AraC Synthesis

Repression by AraC

Detection and Isolation of AraC Protein

Genetics of the Arabinose System

The Eukaryotic Genome

Nucleic Acids and Their Organization in Eukaryotic, Prokaryotic, and Viral Genomes