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Psilocybe mushrooms

المؤلف:  Sue Jickells , Adam Negrusz (Editors)

المصدر:  Clarkes Analytical Forensic Toxicology

الجزء والصفحة:  76

2026-09-20

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Psilocybe mushrooms

 A small quantity (approximately 1 g) of the dried mushrooms is sonicated with methanol (approximately 5 mL) for 10 min. The liquid is removed and reduced in volume at room temperature in an airflow. Psilocybin can be converted into psilocin by heating. This conversion can also occur if the mushrooms are not dried prior to or when they arrive in the laboratory.

Colour test

 With Ehrlich reagent, a violet colour is indicative of psilocybin and psilocin.

Separation and/or identification Direct injection of a solvent extract of psilocybe mushroom onto a GC column converts psilocybin into psilocin by thermal dephosphorylation, and only psilocin is detected. Thus, prior derivatisation is necessary if psilocybin is to be detected. To eliminate sugars that may interfere with derivatisation, 1 ml of acetone is added to the methanolic solution and the mixture allowed to stand for 30 min and then filtered. The filtered solution is then taken to dryness in a stream of air. Pyridine (15 μL), TMS (15 μL) and BSTFA (100 μL) are added and the solution heated at 100C for 30 min. Psilocin is converted to psilocin di-TMS and psilocybin to psilocybin tri-TMS.