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تسجيل

Synthetic Methods for the Preparation of Nucleic Acids

المؤلف:  A. Ravve

المصدر:  Principles of Polymer Chemistry

الجزء والصفحة:  p560-561

2026-02-18

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Synthetic Methods for the Preparation of Nucleic Acids

Over the last 20–30 years, methods were developed to synthesize short deoxyribonucleotide chains [57]. One synthetic procedure can be illustrated as follows:

The bulky triphenylmethyl moiety functions to block the 5 hydroxyl groups and is removed when necessary. The same is true of the acetyl portion that also serves to block the 3 hydroxyl position. The product can be used for further expansion of the chain. Another approach to the syntheses of nucleic acids is to use polymeric supports as in the syntheses of polypeptides. The preparation of protecting groups for attachment to carbon 5 of deoxyribose on the surface of cross-linked polystyrene can be illustrated as follows [57]:

Another protective group that has been used is dimethoxy trityl. The carbon 3 of deoxyribose has been protected with phosphor amide. Benzoyl groups are used to protect the adenine and guanine bases. Lately, in place of cross-linked polystyrene, controlled pore glass supports have become popular. Commercial synthesizing machines are available today for polynucleotide syntheses. These are similar to the synthesizers used in polypeptide syntheses.